參麥、血必凈影響家兔嚴(yán)重燙傷早期血管內(nèi)皮細(xì)胞的研究

摘要： 目的:建立嚴(yán)重燙傷家兔模型,觀察家兔嚴(yán)重燙傷早期血管內(nèi)皮細(xì)胞(vascular endothelial cell,VEC)功能變化及參麥、血必凈注射液對功能的影響,并探討它們對血管內(nèi)皮細(xì)胞功能作用的相關(guān)機制,同時對兩藥療效進行比較。方法:將家兔分成假傷組(8只)、燙傷組(8只)、參麥組(8只)和血必凈組(8組);后3組制成30%體表總面積(TBSA)Ⅲ度燙傷(用92℃水浴燙傷)模型,假傷組采用37℃水浴模擬致傷過程;傷后各組按Parkland公式計算液體量予以復(fù)蘇,同時參麥組每次給予參麥注射液2ml/kg加入等滲鹽水至20ml靜脈滴注,每日3次,連續(xù)2天;血必凈組每次給予血必凈注射液2ml/kg加入等滲鹽水至20ml靜脈滴注,每日3次,連續(xù)2天;另2組同法輸注等容量等滲鹽水。在傷后即刻、6小時、12小時、24小時、48小時時相點抽取家兔頸靜脈血檢測內(nèi)皮素-1、一氧化氮并計算兩者比值水平,同時觀察相應(yīng)時相點家兔的一般情況。結(jié)果:在傷后48小時,假傷組、參麥組、血必凈組ET-1/NO明顯小于燙傷組;參麥組、血必凈組血清ET-1和NO水平均明顯低于燙傷組(P<0.01);參麥組、血必凈組之間比較無統(tǒng)計學(xué)意義(P>0.05);參麥組、血必凈組家兔活躍程度明顯優(yōu)于燙傷組。結(jié)論:參麥注射液和血必凈注射液可能是通過降低嚴(yán)重燙傷早期家兔血清ET-1和NO水平,調(diào)整ET-1/NO比值,對血管內(nèi)皮細(xì)胞起保護作用。
Objective:To establish a rabbit model of severe burns and to observe the influence of Shenmai injection and Xuebijing injection on vascular endothelial cell(vascular endothelial cell,VEC) in rabbit with severe burn at early stage.On the basis of above study,relevant mechanisms of two drugs' function on VEC were discussed and the effects of two drugs were compared.Methods:Rabbits were divided into sham injury group(n = 8),scald group(n = 8),Shenmai group(n = 8) and Xuebijing group(n = 8).The burn models ofⅢdegree scald with 30%TBSA(caused by 92℃water bath scald) were established in later 3 groups.The rabbits in sham injury group were simulated injury with 37℃water bath.After injury,each group was treated with fluid resuscitation according to Parkland formula,while the rabbits in Shenmai group were given intravenous infusion with the solution of 2ml/kg of Shenmai diluted to 20ml with isotonic saline. The infusion was conducted 3 times a day for 2 days;in Xuebijing group,the rabbits were given intravenous infusion with the solution of 2ml/kg of Xuebijing diluted to 20ml with isotonic saline,3 times a day for 2 days;in the rest other two groups,the rabbits were treated with intravenous infusion of the same volume of isotonic saline only.The content of ET - 1 and NO of the sampling carotid blood of the rabbits were determinated and the value of ET - 1/NO was calculated at the time point of 0,6,12,24 and 48h post - injury.Meanwhile,the general conditions of the rabbits at the corresponding time point were observed and recorded.Results:At the time point of 48th h post - injury,ET - 1 / NO ratio of rabbits in sham injury group,Shenmai group and Xuebijng group was obviously less than that in scald group.The level of ET - 1 and NO in Shenmai group and Xuebijng group were significantly lower than that of scald group(P<0.01).But there was no significant difference of level of ET - 1 and NO observed between Shenmai group and Xuebijng group(P>0.05).The degree of rabbit's activity in Shenmai group and Xuebijing group was better than that of scald group.Conclusions:The protective role of Shenmai injection and Xuebijing injection on VEC may be by means of reducing the serum levels of ET - 1 and NO,and adjusting ET -1 / NO ratio in severe scald rabbits at the early stage.