海水浸泡對血管內(nèi)皮功能基因的影響

摘要： 目的血管內(nèi)皮細(xì)胞不僅是機(jī)體循環(huán)系統(tǒng)和組織間的重要屏障,而且具有重要的代謝和內(nèi)分泌功能。本研究主要觀察海水對血管內(nèi)皮功能基因表達(dá)的影響。方法12只Wistar大鼠隨機(jī)均分為腹部開放傷合并海水浸泡組(實(shí)驗(yàn)組)和單純腹部開放傷組(對照組),分別于海水浸泡或術(shù)后2h采集腹部開放傷大鼠小腸系膜血管組織,以實(shí)時(shí)定量PCR芯片法檢測兩組血管內(nèi)皮通透與張力、血管生成、內(nèi)皮激活與損傷等相關(guān)的84個(gè)功能基因的表達(dá)差異。差異表達(dá)大于2倍視為表達(dá)上調(diào)或下調(diào)。結(jié)果與單純腹部開放傷相比,腹部開放傷合并海水浸泡大鼠小腸系膜血管內(nèi)皮77.38%(65/84)的功能基因表達(dá)水平發(fā)生了明顯改變,分別有61.90%(52/84)和15.48%(13/84)的基因表達(dá)水平上調(diào)和下調(diào)。其中,趨化因子Cxcl2表達(dá)水平升高了29.82倍,L-選擇素表達(dá)水平下降了46.59倍。全部血管通透性與張力相關(guān)基因(12/12)和凝血激活相關(guān)基因(6/6)表達(dá)改變大于2倍。結(jié)論海水浸泡廣泛引起腹部開放傷大鼠血管內(nèi)皮重要功能基因表達(dá)改變,內(nèi)皮功能受損。
Objective Sea water is a distinct injurious agent for trauma victim.The course of a trauma might develop differently when the injury is complicated by sea water immersion.Vascular endothelium is the protective layer situated between the blood circulation and tissues,and it possesses metabolic as well as endocrine functions.The purpose of the present study is to investigate the effects of sea water immersion on the expression of functional genes in vascular endothelial cells.Methods Rats were divided into two groups: simple open abdominal injury group and open abdominal injury + sea water immersion group.Two hours after the injury both with or without immersion,the vascular tissue of small intestinal mesentery was harvested,and then the expression levels of a total of 84 functional genes,which were involved in the functions of permeability,tonicity,angiogenesis,endothelial cell activation and injury,in vascular endothelial cells were detected and compared between the two groups by real-time quantitative PCR-array.The expressions were considered as up-or down-regulated when more than 2-folds of expression difference existed.Results Compared with that of the rats in simple open abdominal injury group,the expression levels were markedly changed in 65 of a total of 84 functional genes(77.4%) in the vascular endothelial cells from small intestinal mesentery of the rats in open abdominal injury + sea water immersion group,including 52 genes(61.9%) up-regulated and 13 genes(15.5%) down-regulated.The expression level of chemokine(C-X-C motif) ligand 2 was elevated by 29.8 folds,and that of L-selectin declined by 46.6 folds,respectively.The expression levels of all the genes concerning permeability,vessel tone and thrombin activity changed more than 2 folds.Conclusion Sea water immersion may invoke abnormal gene expression in vascular endothelial cells of small intestinal mesentery in rat.