一氧化碳、甲苯、正壬烷間斷暴露對小鼠全血乙酰膽堿酯酶活性的影響

摘要： 目的:探討不同濃度一氧化碳、甲苯、正壬烷間斷暴露對小鼠全血乙酰膽堿酯酶(AChE)活性的影響。方法:以20日齡雄性Wistar小鼠為實驗對象,隨機(jī)分組,每組12只。將每組小鼠暴露于預(yù)先設(shè)定好的一定濃度某種氣體的密閉動物實驗艙中(用色譜分析法檢測艙室中氣體濃度,保持濃度恒定)。暴露時間為每天4 h,持續(xù)暴露10 d。一氧化碳設(shè)置2個濃度,即57.25、114.50 mg/m3;甲苯設(shè)置3個濃度,即94、188、376 mg/m3;正壬烷設(shè)置3個濃度,即131、262、524 mg/m3。對照組氣體為空氣。第10天暴露完成后,將小鼠處死,取全血測AChE活性。AChE活性的測定采用硫代乙酰膽堿-硫代雙硝基苯甲酸比色測定法[1]。結(jié)果:(1)不同濃度一氧化碳作用對小鼠全血AChE活性的影響:57.25 mg/m3組和114.50 mg/m3組與對照組相比差異均有統(tǒng)計學(xué)意義(P<0.01)。(2)不同濃度甲苯對小鼠全血AChE的影響:94 mg/m3組AChE活性低于對照組,但差異無統(tǒng)計學(xué)意義;188 mg/m3組和376 mg/m3組與對照組相比差異有統(tǒng)計學(xué)意義(P<0.01)。(3)不同濃度正壬烷對小鼠全血AChE活性的影響:3個濃度組AChE活性與對照組相比差異均無統(tǒng)計學(xué)意義。結(jié)論:一氧化碳、甲苯可降低小鼠全血AChE活性,對神經(jīng)系統(tǒng)有一定的抑制作用,并顯示有劑量效應(yīng)關(guān)系趨勢;本實驗濃度的正壬烷對小鼠全血AChE活性影響不明顯。
Objective:To explore the effects of respective discontinous exposure to carbon monoxide(CO)、methybenzene(C7H8) and o-nonane (nC9H20) of different concentration on the activities of the whole blood AChE in mice.Methods:Male Wistar mice whose age were 20-day old were randomly divided into groups.There were 12 mice in each group who were exposed to a kind of gas with invariably concentration in animal experiment cabin closed tightly for 4 hours every day and the exposure was continued 10 days.The method of chromatographic analysis was used to control gas concentration constancy in cabin.CO was setted 2 different concentrations: 57.25 mg/m3 and 114.50 mg/m3.And C7H8,3 different concentrations: 94 mg/m3,188 mg/m3 and 376 mg/m3.And nC9H20,3 different concentrations: 131 mg/m3,262 mg/m3 and 524 mg/m3.The gas in control group was air.The activities of whole blood in mice executed on the 10th day of exposure were measured by ASCh-DTNB method.Result:(1) In CO experiments: the activities of the whole blood AChE in mice in 57.25 mg/m3 group and in 114.5 mg/m3 group were significantly decreased compared with those in the control group(P<0.01).(2) In C7H8 experiments,the activities of AchE in 94 mg/m3 group were not statistically different compared with those in the control group.While C7H8 of either 188 mg/m3,376 mg/m3 concentration can statistically decreased the activities of AChE compared with the control group(P<0.01).(3) In nC9H20 experiments,the activities of AChE in 3 concentration groups were no significant differences compared with those in the control group.Conclusion: CO and C7H8 can lower the activities of the whole blood AChE in mice and inhibit the function of nervous system and tend to dosage-effect relation between them.In addition,this study indicated that nC9H20 could not affect on activities of the whole blood AchE in mice.