四肢火器傷受紫外線照射后堿性成纖維細(xì)胞生長(zhǎng)因子的表達(dá)

摘要： 目的研究四肢火器傷受不同劑量短波紫外線(UVC)照射后傷口肉芽組織中堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)表達(dá)變化。方法建立兔四肢軟組織火器傷模型,UVC30mJ/cm2和60mJ/cm2分別進(jìn)行傷道內(nèi)照射。采用免疫組化和原位雜交的方法觀察組織中bFGF的表達(dá)。結(jié)果致傷照射后7～21天,60mJ/cm2照射組和30mJ/cm2照射組bFGF在mRNA水平與蛋白水平的表達(dá)均高于對(duì)照組(P<0.05),以60mJ/cm2照射組更為顯著;照射后21天,60mJ/cm2照射組bFGF的mRNA水平顯著降低,低于30mJ/cm2照射組(P<0.05)。結(jié)論在傷口愈合早期UVC照射能促進(jìn)bFGF的表達(dá),以60mJ/cm2的作用更顯著。但不同劑量UVC促進(jìn)bFGF表達(dá)的時(shí)相性有所不同。
Objective To investigate the effect of different irradiation doses of ultraviolet C ray(UVC)on the expression of basic fibroblast growth factor(bFGF)in granulation tissue of gunshot wound in rabbit limbs.Methods After giving 30mJ/cm2 or 60mJ/cm2 UVC to the gunshot wound of soft tissue of limbs,the expression of bFGF was observed both at the mRNA level and the protein level by the methods of in situ hybridization and immunohistochemistry,respectively.Results On the 7th day after UVC irradiation,the expression of bFGF in UVC-treated groups was significantly higher than that in control group(P<0.05)at both the mRNA and the protein levels,especially in the 60mJ/cm2 group.While on the day 14 after UVC treatment,the bFGF protein and mRNA levels in 60mJ/cm2 group decreased rapidly and were lower than that in the other two groups(P<0.05).Conclusion In the initial stage of wound healing,the treatment of UVC irradiation promoted the expression of bFGF at different dose levels,but the time phases of the promotion effect of different doses were diverse.