Evaluation of Genotoxicity of Abrus mollis Hance with Single-cell Gel Electrophoresis Assay

摘要： [Objective] This study aimed to evaluate the genotoxicity of Abrus mollis Hance by using single cell gel electrophoresis.[Method] Forty mice were divided into five groups randomly,including positive control group(cyclophosphamide group),negative control group(physiological saline group),high-dose A.mollis Hance group(30 g/kg),moderate-dose A.mollis Hance group(20 g/kg) and low-dose A.mollis Hance group(10 g/kg).Tail DNA% and Tail Moment of mouse liver,kidney,lung and testicular cells were analyzed by using single cell gel electrophoresis assay,to investigate the effect of A.mollis Hance on DNA in mouse cells.[Result] Compared with positive control group,Tail DNA% and Tail Moment of mouse liver,kidney,lung and testicular cells in A.mollis Hance groups were significantly lower(P<0.01).Compared with negative control group,Tail DNA% and Tail Moment of mouse liver,kidney,lung and testicular cells in high-dose A.mollis Hance group were significantly lower(P <0.01),while the other A.mollis Hance groups showed no statistically significant difference(P>0.05).[Conclusion] A.mollis Hance has no damage effect on DNA in mouse cells within this experimental dose range. （共3頁）