Selection of the Mutants with High Hydroquinone Degradation Ability of Serratia Marcesscen by Plasma Mutation

摘要： <正> In this study,an efficient way by plasma induced mutation was applied to improvethe hydroquinone degradation capacity of Serratia marcescens AB 90027(SM27).The resultsshowed that combined with the selection of hydroquinone tolerance,the mutant with high hydroquinonedegradation ability induced by plasma could be achieved.The best dose for plasmamutation was 15s,which showed a 47.0% higher positive mutation ratio.Besides,the aimedmutant was markedly different from the parent strain(SM27)in colonial traits while cultivatedon Kings media.Finally,the hydroquinone degradation ratio reached 70.5% using the inducedmutant strain with 1500 mg/L hydroquinone(HQ)after 15 days of cultivation as the selectiveconditions;however,it was only 46.7% for SM27.The improvement of the degradation capacityby the induced mutant with a high concentration of HQ selection was attributed to its fastergrowth and higher hydroquinone tolerance compared with that of the parent strain. （共4頁(yè)）